Refine your search
Collections
Co-Authors
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Maslachah, Lilik
- Phenotypic Approach Artemisinin Resistance in Malaria Rodent as in vivo Model
Abstract Views :149 |
PDF Views:0
Authors
Affiliations
1 Department of Basic Medicine, Veterinary Pharmacy Laboratory, Airlangga University Surabaya, ID
2 Department of Pathology, Airlangga University Surabaya, ID
3 Department of Veterinary Anatomy, Airlangga University Surabaya, ID
4 Department of Veterinary Pathology, Airlangga University Surabaya, ID
1 Department of Basic Medicine, Veterinary Pharmacy Laboratory, Airlangga University Surabaya, ID
2 Department of Pathology, Airlangga University Surabaya, ID
3 Department of Veterinary Anatomy, Airlangga University Surabaya, ID
4 Department of Veterinary Pathology, Airlangga University Surabaya, ID
Source
Veterinary World, Vol 10, No 7 (2017), Pagination: 790-797Abstract
Aim: The aim of this study is to prove the development of artemisinin resistance phenotypically in malaria rodent as an in vivo resistance development model in humans.
Materials and Methods: Plasmodium berghei was infected intraperitoneally in mice, then artemisinin was given with “4-day-test” with effective dose (ED) 99% dose for 3 days which begins 48 h after infection (D2, D3, and D4). Parasite development was followed during 5th until 10th days of infection. After parasitemia >2% of red blood cell which contains parasites on 1 mice, that mice were used as donor to be passaged on the new 5 mice. After that, parasitemia was calculated. ED50 and ED90 were examined with parasite clearance time (PCT), recrudescence time (RT), and also morphology development examination of intraerythrocytic cycle of P. berghei with transmission electron microscope.
Results: Among the control group compare with the treatment group showed significant differences at α=0.05 on 5th day (D5) until 10th day (D10). The control group of 4th passage (K4) with passage treatment group of 4th passage (P4) on the 10th days (D10) post infection showed no significant differences in the α=0.05. The average percentage of inhibition growth was decreasing which is started from 5th to 10th day post infection in P1, P2, P3, and P4. On the development of P. berghei stage, which is given repeated artemisinin and repeated passage, there was a formation of dormant and also vacuoles in Plasmodium that exposed to the drug.
Conclusion: Exposure to artemisinin with repeated passages in mice increased the value of ED50 and ED90, decreased the PCT and RT and also changes in morphology dormant and vacuole formation.
Keywords
Artemisinin, Parasite Clearance Time, Phenotypic, Plasmodium berghei , Recrudescence Time, Resistance.Full Text
- Adjuvant Therapy of Syzygium Cumini Leaf and Fruit Extract Nanoparticles to Histopathological Changes of Mice Organ with Malaria
Abstract Views :74 |
PDF Views:0
Authors
Lilik Maslachah
1,
Thomas V Widiyatno
2,
Nusdianto Triakoso
3,
Suwarno
4,
Koesnoto P
5,
Nanda Ayu Narulita
6,
Mahendra Pujiyanto
6,
Zerlinda Dyah Ayu
6,
Dita Nurkurnia Putri
6
Affiliations
1 Department of Basic Veterinary Medicine, Universitas Airlangga, Surabaya,, ID
2 Department of Veterinary Pathology, Universitas Airlangga, Surabaya,, ID
3 Department of Veterinary Pathology, Universitas Airlangga, Surabaya, ID
4 Department of Veterinary Microbiology, Universitas Airlangga, Surabaya,, ID
5 5Department of Husbundary, Universitas Airlangga, Surabaya,, ID
6 Student Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya,, ID
1 Department of Basic Veterinary Medicine, Universitas Airlangga, Surabaya,, ID
2 Department of Veterinary Pathology, Universitas Airlangga, Surabaya,, ID
3 Department of Veterinary Pathology, Universitas Airlangga, Surabaya, ID
4 Department of Veterinary Microbiology, Universitas Airlangga, Surabaya,, ID
5 5Department of Husbundary, Universitas Airlangga, Surabaya,, ID
6 Student Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya,, ID
Source
Research Journal of Pharmacy and Technology, Vol 15, No 1 (2022), Pagination: 389-394Abstract
Plasmodium infection can cause serious complications such as damage to internal organs. The purpose of this study was determining the effect of nanoparticles adjuvant therapy of Syzygium cumini leaf and fruit extract to histopathological changes of mice organ with malaria. Methods: The study used 70 Swiss Albino mice, weighing 20g-30g, 2.5 months old. The study was divided into 7 treatment groups as follows K0 : not infected, K+: infected but untreated, K1: infected and treat with chloroquine, K2: infected and treated with Syzygium cumini leaf extract, K3: infected and treated with Syzygium cumini fruit extract, P1: infected and treated with nanoparticles Syzygium cumini leaf extract, P2: infected and treated with combination of nanoparticles leaf extract of Syzygium cumini and chloroquine, P3: infected and treated with nanoparticles Syzygium cumini fruit extract, P4: infected and treated with combination of nanoparticles fruit extract of Syzygium cumini and chloroquine. The infection dose was 1x10-5 Plasmodium berghei in 0.2 ml. Treatment was done for 4 days, 24 hours after infection. Data were analyzed by Kruskal Wallis and continued with the Mann Whitney test. The results showed that the most severe organ damage was in the K+ group and the lightest was the K0 group. The P2 and P4 groups showed a decrease in organ damage which was not significantly different from the K0 group (p> 0.05). Meanwhile, K +, K1, K2, K3, P1 and P3 groups showed significant differences (p> 0.05) with K0. Conclusion: nanoparticles extract of Syzygium cumini leaf and fruit as an adjuvant therapy can reduce liver, kidney, lung and brain damage of mice infected with Plasmodium berghei.Keywords
Malarial Infection, Organ Damage, Nanoparticles, Syzygium cumini.
References
- WHO. World Malaria Report. ISBN 978-92-4-156572-1. 2019;45-56.
- Good MF, Doolan DL. Malaria Vaccine Design : Immunological Consideration. Immunity.2010; 33(4): 555-566.
- Sumbele I, Theresa NA, Samje M, Thomas N, Titanji VPK, Manha NE. Haematological Changes and Recovery Associated with Treated and Untreated Plasmodium falciparum Infection in Children in the Mount Cameroon Region. Clinical Medicine and Research.2010; 2(9): 143-151.
- Okochi and Okpuzor. Micronutrients as Therapeutic Tools in the Management of Sickle Cell Disease, Malaria and Diabetes. African Journal of Food Agriculture Nutrition and Development 2011; 4(13):1-6.
- Becker K, Tilley L, Jonathan L, Roberts DV, Ginsburg H. Oxidative Stress in Malaria Parasite Infected Erythrocyte: Host-Parasite Interaction. International Journal for Parasitology. 2004; 34(2): 163-189.
- Percario S, Moreira BAQ, Gomes MES, Ferreira ACM, Goncalves PSOC, Laurindo TC, Vilhena MF, Dolabela and Green MD. Review Oxidative Stress in Malaria. International Journal of Molecular Sciences. 2012;13(12):16346-16372.
- Mathur, Prateek AU, Jha, Swati AU, Ramteke, Suman AU, Jain, Narendra. Pharmaceutical Aspects of Silver Nanoparticles. Artificial Cell.2017; 46(1):1- 12.
- Zhang , LL and Lin YM. Antioxidant Tannins from Syzygium cumini fruit. African Journal of Biotechnology. 2009; 8(10): 2301-2309.
- Maslachah L, Sugihartuti R. Potency Syzygium cumini L as Adjuvant Therapy on Mice Model Malaria. Iraqi Journal of Veterinay Sciences. 2018; 32(1): 73-80.
- Craig, Alister G, Georges E, Grau, Chris Janse, James W, Kazura, Milner, John W, Barnwell, Gareth Turner, Jean Langhorne. The Role of Animal Models for Research on Severe Malaria. PLoS. Pathog. 2012; 8(2): e1002401.
- Iribhogbe OI, Agbaje EO, Oreagba LA, Aina O dan Ota AD. Oxidant versus Antioxidant Activity in Malaria: Role of Nutritional Therapy. Journal of Medical Sciences. 2012;12(7):229-233.
- Tadesse S, Wubneh Z. Antimalarial Activity of Syzygium guineense During Early and Established Plasmodium infection in Rodent Models. Complementary and Alternative Medicine. 2017; 17(1): 1-7.
- George BO, Okpoghono J, Osioma E, Aina OO. Changes in Oxidative Indices in Plasmodium berghei Infected Mice Treated with Aqueous Extract of Aframomum sceptrum. Frontiers in Science. 2012; 2(1): 6-9.
- Schönenberger MJ and Kovacs WJ. Hypoxia Signaling Pathways: Modulators of Oxygen-Related Organelles. Frontiers in Cell and Developmental Biology. 2015; 3(42):1-19.
- Adwas AA, Elsayed ASI, Azab AE, Quwaydir FA. Oxidative Stress and Antioxidant Mechanisms in Human Body. Journal of Biotechnology. 2019; 6(1):43‒47.
- Olivier M, Van Den Ham K, Shio MT, Kassa FA and Fougeray S. Malarial Pigment Hemozoin and the Innate Inflammatory Response. Frontiers in Immunology. 2014; 5(25):1-10.
- De Souza MC, Padua TA, and Henriques GA. Multiple Organ Dysfunction During Severe Malaria: The Role of Inflammatory Response in Current Topics in Malaria. Intech. 2016; 5: 1-22
- Parhizgar AR and Tahghighi A. Introducing New Antimalarial Analogues of Chloroquine and Amodiaquine : A Narrative Review. Iranian Journal of Medical Sciences.2017; 42(2):115-128.
- Kavishe RA, Koenderink JB and Alifrangis M. Oxidative Stress in Malaria and Artemisinin Combination Therapy: The FEBS Journal. 2017; 284(16): 2579-2591.
- Varo R, Crowley VM, Sitoe A, Madrid L, Serghides L, Kain KC and Bassat Q. Adjunctive Therap For Severe Malaria: A Review and Critical Apprasial. Malaria Journal.2018; 17(47): 1-18.
- Prato M, Gallo V, Giribaldi G, Aldieri E, Arese P. Role of the NF-κB Transcription Pathway in the Haemozoin- and 15-HETE-Mediated Activation of Matrix Metalloproteinase-9 in Human Adherent Monocytes. Cell Microbiol. 2010;12(12):1780-91.
- Prato and Giribaldi. New Perspectives for Adjuvant Therapy in Severe Malaria. Journal Bacteriology & Parasitology. 2012; 3(5): 1-2.